Utilization of a simple and fast electroanalytical method with an unmodified boron-doped diamond electrode for taxifolin sensing in food supplement samples

Abstract
Abstract\r\n Using an unmodified boron-doped diamond electrode, an electrochemical sensor was established for the determination \r\nof taxifolin through square-wave voltammetry. Cyclic voltammetry measurements conducted in a 0.1 M HClO4 solution \r\nshowed that taxifolin exhibits irreversible behavior with diffusion control, marked by distinct oxidation peaks at approxi\r\nmately + 1.04 V (PA1) and + 1.44 V (PA2) versus Ag/AgCl. Furthermore, the pH as well as the supporting electrolyte had an \r\neffect on the oxidation process. The quantification of taxifolin was based on its two anodic peaks. The optimized method \r\nyielded a current response that was linearly proportional to taxifolin concentration, ranging from 2.5 to 50.0 μg mL−1 \r\n(8.2 × 10–6 to 1.6 × 10–4 M), with detection limits of 0.72 μg mL−1 (2.4 × 10–6 M) for PA1 and 0.63 μg mL−1 (2.1 × 10–6 M) \r\nfor PA2 in HClO4. The method was successfully applied for the evaluation of taxifolin in a food supplement, thereby \r\ndemonstrating its practical applicability. This study is among the first to utilize both oxidation peaks of taxifolin in elec\r\ntroanalytical measurements. Comparisons between the results obtained using this technique and those from the UV–Vis \r\nspectrometry method confirmed the accuracy of the approach.\r\n Keywords Taxifolin · Boron-doped diamond electrode · Voltammetric sensing · Food supplemen

Author
Hoshyar Saadi Ali

DOI
https://doi.org/10.1007/s13197-025-06456-z

ISSN

Publish Date: 10-Dec-2025