Genome-wide analysis of the BoBZR1 family genes and transcriptome analysis in Brassica oleracea

Abstract
The BRASSINAZOLE-RESISTANT 1 genes play a crucial role as key regulators in Brassinosteroid (BR) \r\nsignaling, which affects various plant developmental and stress-responsive aspects. Understanding \r\nregulatory mechanisms via BZR1 in modulating target genes has become a main point in research \r\non plant BR signaling networks. Despite this, the BZR1 functioning in B. oleracea is not elucidated. \r\nA complete genome-wide analysis identified 12 BZR1 genes in B. oleracea, categorized into three \r\ngroups based on their gene motif and structural features. These BoBZR1s were found on eight different \r\nchromosomes. Synteny analysis between B. oleracea, Arabidopsis, and potato provided perception \r\ninto their evolutionary characteristics. Promoter regions of BoBZR1 family genes in B. oleracea have \r\nshown specific cis-elements associated with hormones, stress, and plant development. The expression \r\nanalysis toward cuticular wax biosynthesis revealed that BoBZR1-1, BoBZR1-6, BoBZR1-7, and \r\nBoBZR1-10 were upregulated in response to cuticular wax biosynthesis. Differential expressions \r\nof BoBZR1 genes were observed for all seven different tested tissues. The whole study involved \r\nsystematic characterization of the BoBZR1 family, and expression patterns, in BR signaling and its \r\nextensive involvement in developmental processes in B. oleracea. Results establish a theoretical \r\nfoundation for deeper investigation of BoBZR1 structure and functions in B. oleracea, specifically \r\ntoward regulating plant stress.\r\n

Author
Tayebeh Ahmadi

DOI
https://doi.org/10.1038/s41598-025-99487-7

ISSN

Publish Date: 2025-05-03