Investigate Freshwater Algae Extract\'s Efficacy in Treating Diabetes Ulcers and Its Anti-Staphylococcal Properties
Abstract
Background: Infection of diabetic foot ulcer is very common and leads in 20% of cases to amputation. \r\nAntibiotic-resistant Staphylococcus aureus is the main cause of severe infection. Antibiotic resistance \r\nis a major challenge to the global health system. This work aimed to investigate the antibacterial efficacy \r\nof some algae extracts against Staphylococcus aureus isolated from diabetic foot ulcers. \r\nMethods: freshwater river samples were collected to isolate the algae, and PCR was used for \r\nidentification. The ethanol, water, and ethyl acetate extract of these algae were prepared and analyzed \r\nusing high-performance liquid chromatography-mass spectrometry to determine the key components \r\nthat have antibacterial properties. The antibacterial activity of these extracts against S. aureus was \r\ndetermined by broth dilution and well diffusion methods. \r\nResults: Chlorella vulgaris and Anabaena flos-aquae were isolated from freshwater river and identified \r\nby PCR. Anabaena flos-aquae has a greater antibacterial efficacy against Staphylococcus aureus in \r\ncomparison to Chlorella vulgaris, and the ethanolic extract demonstrated superior outcomes compared \r\nto the aqueous and ethyl acetate extracts. The MS spectrum of both algae had a very similar pattern, but \r\nthe frequency of detected peaks was different \r\nConclusion: Ethanolic extract of A. flos-aquae and Chlorella vulgaris can be suggested to treat and \r\ncontrol diabetic foot ulcer infection caused by S. aureus. Further studies are required to explore the full \r\npotential of these algae safely and extensively.
Author
Alwan Qader Ahmed, Nyan Jasim Mohammed, Zean Fetehallah Zefenkey, Shilan Farhad Mamand, Sahar Hassannejad, Abdullah Othman Hassan, Rawaz Rizgar Hassan
ISSN
2322-3480
Publish Date: 2024-04-01