Antioxidant, α-glucosidase, antimicrobial activities chemical composition and in silico analysis of eucalyptus camaldulensis dehnh

Abstract
Throughout history, medicinal plants have been the primary source for preventing and \r\ntreating infectious diseases and other health issues. The flowering plant Eucalyptus camaldulensis, \r\nalso called river red gum, is a member of the Myrtaceae family and has numerous traditional uses. The \r\nobjectives of the present study were to identify the essential oil components of Eucalyptus \r\ncamaldulensis using Gas Chromatography Mass Spectrometry (GCMS), and to determine the \r\nantioxidant, antidiabetic, and antibacterial activities of ethanol, aqueous, and essential oil extracts from \r\nE. camaldulensis leaves. Addtionally, the essential oil constituents that were identified underwent an\r\nin silico analysis. The efficacy of various extracts in combat pathogens and free radicals was assessed \r\nthrough the utilization of the 1,1-Diphenyl-2-Picryl Hydrazyl (DPPH), ferric reducing antioxidant power (FRAP), α-glucosidase inhibition, and disk diffusion methods. In terms of radical scavenging, \r\nreducing power, and α-glucosidase inhibitory activity, the essential oil showed strong antioxidant \r\nactivity at 84.01 %, 20.1 mmol/g, and 78.2 %, respectively. The essential oil showed a potent \r\nantimicrobial action against Staphylococcus aureus and Pseudomonas aeruginosa, with 12 and 14 mm\r\ninhibitions, respectively, which were higher than ampicillin\'s 9 and 6 mm inhibitions, respectively. The \r\nGCMS analysis showed that the following chemicals were the most common: cis-11-hexadecenal (10.2%), \r\ntrans-13-octadecenoic acid (9.5%), and 6-Octadecenoic acid, methyl ester, (Z)-(8.8%). The αglucosidase enzyme was targeted in a docking study to investigate the antidiabetic properties of the 42 \r\nphytochemicals found in the essential oil extract. The compound, namely 5.alpha.-Androstan-16-one, \r\nshowed the highest binding affinities of −8.6 Kcal/mol during the docking screening of the 42 \r\nidentified phytochemicals against α-glucosidase. These two compounds show potential as competitive \r\nα-glucosidase inhibitors. E. camaldulensis will be a particularly useful source to improve health and \r\nfight communicable and non-communicable diseases. Nonetheless, human evaluations of E. camaldulensis safety and effectiveness are necessary, and more well-planned clinical trials are needed \r\nto confirm our in vitro and in silico findings.\r\n

Author
Saber Wasman Hamad

DOI
https://www.aimspress.com/article/doi/10.3934/biophy.2024015

Publisher
AIMS Biophysics

ISSN

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