Spectrophotometric Determination of Loperamide Hydrochloride by Ion-Pair Formation with Rose Bengal Reagent

Abstract
A simple and accurate spectrophotometric technique has been presented for determining the presence of loperamide hydrochloride. The approach involves forming an ion-pair association complex with rose Bengal (RB), which results in a colored\r\nproduct that is analyzed spectrophotometrically at 568 nm. The validity of Beer’s law was established in the range of 0.5–10\r\nμg/L. The proposed method is specifc to the analysis of loperamide and can diferentiate it from its pharmaceutical products, which can be detected using HPLC. The conditions for the method were optimized, and the technique was successfully\r\nused to analyze the drug in both pure form and dosage forms. The study establishes a low limit of detection of 0.025 and a\r\nlimit of quantifcation of 0.082. The method provides a reliable and sensitive approach for the determination of loperamide\r\nhydrochloride in pharmaceutical formulations and can be used as an alternative to other analytical methods. The results\r\ndemonstrate the potential for using this method in routine quality control analysis.

Author
Diyar Salahuddin Ali

DOI
https://doi.org/10.1007/s12161-023-02498-1

Publisher
Food Analytical Methods

ISSN
1936-9751

Publish Date: