Association study of Bif‑1 gene expression with histopathological characteristics and hormone receptors in breast cancer
Abstract
Abstract\r\nBackground: Breast cancer is a heterogeneous disease that has various clinical outcomes. Bax-interacting factor-1\r\n(Bif-1) is a member of the endophilin B family that generates the pro-apoptotic BCL2-Associated X (BAX) protein in\r\nresponse to apoptotic signals. Lack of Bif-1 inhibits the intrinsic pathway of apoptosis and enhancements the risk\r\nof tumor genesis. The present study aimed to investigate the relationship between hormone receptors (ER, PR, and\r\nHER2) status and diferent levels of Bif-1 gene expression in breast cancer patients.\r\nMethods: Bif-1 gene expression was evaluated in 50 breast cancer tumors and 50 normal breast mammary tissues\r\nusing the SYBR Green real-time RT-PCR technique. Multivariate and univariate analyses were used to appraise the\r\nrelationship between the prognostic significance of the Bif-1 gene using SPSS software. In this study, the Bif-1 was\r\nselected as a candidate for a molecular biomarker and its expression status in breast cancer patients with hormone\r\nreceptors (ER, RR, and HER2) compared to patients without these hormone receptors.\r\nResults: The study showed that the relative expression of the Bif-1 gene in tissues of patients with hormone receptors in breast cancer compared to those without hormone receptors was not statistically significant. The expression\r\nlevels of the Bif-1 gene in different groups were evaluated for hormone receptor status. No significant relationship was\r\nfound between the Bif-1 gene expression and hormone receptors (ER, PR, and HER2) (p>0.05).\r\nConclusion: Bif-1 gene expression may be a useful prognostic marker in breast cancer.\r\nKeywords: Breast cancer, Bif-1, Gene expression, Real-time PCR, Biomarker
Author
Kazhaleh Mohammadi, Mahdieh Salimi, S. Abdolhamid Angaji, Arthur Saniotis and Foroozandeh Mahjoobi
DOI
https://doi.org/10.1186/s12905-022-02075-4
Publisher
BMC Women’s Health
ISSN
1472-6874
Publish Date: