Evaluation cell surface hydrophobicity of four potential probiotics Lactic acid bacteria's isolated from local dairy products
DOI:
https://doi.org/10.59480/phahs.v1i2.25Keywords:
Probiotic, adherence. xylene, hexadecane, chloroform, ethyl acetate.Abstract
The purpose of this study was to investigate the cell surface hydrophobicity of four Lactic acid bacteria (LAB) strains isolated from various traditional and industry fermented yogurt and cheese sources based on the biochemical tests. The determining cell surface hydrophobicity is a critical step in selecting LAB strains with probiotic qualities and is one of the most essential elements regulating LAB's particular adherence to abiotic surfaces, as well as one of the most critical factors controlling LAB's absorption and destruction of hydrophobic organic matter. In current study, the four LAB strains had high cell surface hydrophobicity to the four separate hydrocarbon solvents xylene, hexadecane, chloroform, ethyl acetate determined via spectrophotometer at 450 nm. The highest hydrophobicity score was determined for LB3 for chloroform, n- hexadecane, ethyl acetate and xylene at 66.51, 38.23, 33.67 and 24.18% respectively. LB3 demonstrated promising cell surface characteristics, suggesting that it could be used as an indigenous probiotic
References
Bandyopadhyay, B., Das, S., Mitra, P. K., Kundu, A., Mandal, V., Adhikary, R., & Mandal, N. C. (2022). Characterization of two new strains of Lactococcus lactis for their probiotic efficacy over commercial synbiotics consortia. Brazilian Journal of Microbiology, 1-18. https://doi.org/10.1007/s42770-022-00685-6
Bhushan, B., Sakhare, S. M., Narayan, K. S., Kumari, S., Mishra, V., & Dicks, L. M. (2021). Characterization of riboflavin-producing strains of Lactobacillus plantarum as potential probiotic candidate through in vitro assessment and principal component analysis. Probiotics & Antimicrobial Proteins, 13, 453–467. https://doi.org/10.1007/%20s12602-020-09696-x
Carey, A. B., Ashenden, A., & Köper, I. (2022). Model architectures for bacterial membranes. Biophysical reviews, 1-33.
Chauvière, G.; Coconnier, M.-H.; Kerneis, S.; Darfeuille-Michaud, A.; Joly, B., .& Servin, A.L. (1992). Competitive exclusion of diarrheagenic Escherichia coli (ETEC) from human enterocyte-like Caco-2 cells by heat-killed Lactobacillus. FEMS Microbiol. Lett., 91, 213–217 https://doi.org/10.1111/j.1574-6968.1992.tb05211.x
Clark, D. P., & Pazdernik, N. J. (2013). Molecular biology. Elsevier. Chapter e10 - Cell Division and DNA Replication https://doi.org/10.1016/B978-0-12-378594-7.00044-5
Frelet-Barrand, A. (2022). Lactococcus lactis, an Attractive Cell Factory for the Expression of Functional Membrane Proteins. Biomolecules, 12(2), 180. https://doi.org/10.3390/biom12020180
Khojah, E., Gomaa, M. S., Elsherbiny, E. G., Elawady, A., & Darwish, M. S. (2022). The In Vitro Analysis of Postbiotics in Functional Labneh to Be Used as Powerful Tool to Improve Cell Surfaces Properties and Adherence Potential of Probiotic Strains. Fermentation, 8(3), 122. https://doi.org/10.3390/fermentation8030122
Klopper, K. B., Deane, S. M., & Dicks, L. M. (2018). Aciduric strains of Lactobacillus reuteri and Lactobacillus rhamnosus, isolated from human feces, have strong adhesion and aggregation properties. Probiotics and Antimicrobial Proteins, 10(1), 89–97. https:// doi.org/10.1007/s12602-017-9307-5 https://doi.org/10.1007/s12602-017-9307-5
Samet, K. and Icen, T . (2022). Screening of Potential Probiotic Properties of Lactobacillus and Lactococcus strains. Vol. 17 (1) January (2022) Res. J. Biotech
Kos, B.; Šuškovi´c, J.; Vukovi´c, S.; Šimpraga, M.; Frece, J.,& Matoši´c, S. (2003). Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92. J. Appl. Microbiol., 94, 981–987. https://doi.org/10.1046/j.1365-2672.2003.01915.x
Kumari, M., Patel, H. K., Kokkiligadda, A., Bhushan, B., & Tomar, S. K. (2022). Characterization of probiotic lactobacilli and development of fermented soymilk with improved technological properties. LWT, 154, 112827. https://doi.org/10.1016/j.lwt.2021.112827
Liu, W., Li, W., Zheng, H., Kwok, L. Y., & Sun, Z. (2022). Genomics divergence of Lactococcus lactis subsp. lactis isolated from naturally fermented dairy products. Food Research International, 155, 111108. https://doi.org/10.1016/j.foodres.2022.111108
Marin M.L., Benito Y., Pin C., Fernandez M.F., Garcia M.L., Selgas M.D. and Casas C., (1997). Lactic acid bacteria: Hydrophobicity and strength of attachment to meat surfaces, Lett Appl Microbiol, 24(1), 14-18 https://doi.org/10.1046/j.1472-765X.1997.00334.x
Ołdak, A., Zielińska, D., Łepecka, A., Długosz, E., & Kołożyn-Krajewska, D. (2020). Lactobacillus plantarum strains isolated from Polish regional cheeses exhibit anti-staphylococcal activity and selected probiotic properties. Probiotics and antimicrobial proteins, 12(3), 1025-1038. https://doi.org/10.1007/s12602-019-09587-w
Prabhurajeshwar, C., & Chandrakanth, K. (2019). Evaluation of antimicrobial properties and their substances against pathogenic bacteria in-vitro by probiotic Lactobacilli strains isolated from commercial yoghurt. Clinical Nutrition Experimental, 23, 97-115. https://doi.org/10.1016/j.yclnex.2018.10.001
Reiner, K. (2010). Catalase test protocol. American society for microbiology, 1-9.
Sedighi M., Zahedi Bialvaei A., Hamblin M.R., Ohadi E., Asadi A., Halajzadeh M., Lohrasbi V., Mohammadzadeh N., Amiriani T., Krutova M., Amini A. ,& Kouhsari E., (2019). Therapeutic bacteria to combat cancer; current advances, challenges and opportunitie, Cancer Med, 8(6), 3167-3181 https://doi.org/10.1002/cam4.2148
Sharma, A., Lavania, M., Singh, R., & Lal, B. (2021). Identification and probiotic potential of lactic acid bacteria from camel milk. Saudi Journal of Biological Sciences, 28(3), 1622-1632. https://doi.org/10.1016/j.sjbs.2020.11.062
Song, A. A. L., In, L. L., Lim, S. H. E., & Rahim, R. A. (2017). A review on Lactococcus lactis: from food to factory. Microbial cell factories, 16(1), 1-15. https://doi.org/10.1186/s12934-017-0669-x
Yadav R., Puniya A.K. ,& Shukla P., (2016). Probiotic Properties of Lactobacillus plantarum RYPR1 from an Indigenous Fermented Beverage Raabadi, Front Microbiol, 7, 1683. https://doi.org/10.3389/fmicb.2016.01683
Yerlikaya O., (2019). Probiotic potential and biochemical and technological properties of Lactococcus lactis ssp. lactis strains isolated from raw milk and kefir grains, J Dairy Sci, 102(1), 124- 134. https://doi.org/10.3168/jds.2018-14983
Yi, R., Tan, F., & Zhao, X. (2019). Probiotic properties of Lactobacillus strains from traditional fermented yogurt in Xinjiang. In E3S Web of Conferences (Vol. 131, p. 01121). EDP Sciences. https://doi.org/10.1051/e3sconf/201913101121
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